Protocols and Tutorials - Mississippi Genome Exploration Laboratory - MGEL - Daniel G. Peterson - Genomics, Bioinformatics, Cytogenetics, Molecular Biology - Loblolly Pine, Pinus taeda, Gymnosperms, Cotton, Plant & Animal Pathogens/Pests, Cattle

PROTOCOLS & TUTORIALS

As a service to fellow scientists/students, this website contains several detailed scientific protocols and slide show tutorials introducing biological concepts.

To access the "PDF" files, Adobe Acrobat Reader must be installed on your computer.  If you don't have Adobe Acrobat Reader, you can download the program for free from the Adobe website

PROTOCOLS - Utilized and/or developed at MGEL

Isolation of nuclear DNA from plants (PDF) - Revised 5/07/2005

Construction of plant bacterial artificial chromosome libraries (PDF)

Siliconizing glassware (PDF)

TUTORIALS - We have prepared several tutorials designed to help interested individuals understand specific biological concepts. 

Fluorescence in situ hybridization (FISH) (PDF) - Presents the various steps in FISH using pachytene chromosomes as substrates

Cot Analysis: An Overview (PDF) - A review of the steps in a traditional DNA renaturation kinetics study (Cot analysis)

Preparing Solutions and Making Dilutions (PDF) - A useful "how to guide" for students of all ages